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Literature summary for 3.1.1.64 extracted from
- Identification of a novel palmitylation site essential for membrane association and isomerohydrolase activity of RPE65 (2009), J. Biol. Chem., 284, 3211-3218.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the Cys-112 palmitylation site is essential for the membrane association and activity of RPE65 | Homo sapiens | |
| additional information | the Cys-112 palmitylation site is essential for the membrane association and activity of RPE65 | Bos taurus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in 293A-LRAT cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C106A | the mutation does not affect the palmitylation status of the fragment 98-118, the mutant remains associated with the membrane. The mutant has an intact enzymatic activity similar to that of wild type enzyme | Homo sapiens |
| C112A | the mutation abolishes palmitylation in fragment 98-118 and dissociates RPE65 from the membrane. The mutant has no enzymatic activity | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Homo sapiens | 16020 | - |
| membrane | - |
Bos taurus | 16020 | - |
| microsome | - |
Homo sapiens | - |
- |
| microsome | - |
Bos taurus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
x * 61000, estimated from amino acid sequence | Homo sapiens |
| 61000 | - |
x * 61000, estimated from amino acid sequence | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| retinal pigment epithelium | - |
Homo sapiens | - |
| retinal pigment epithelium | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| all-trans-retinyl ester + H2O | - |
Homo sapiens | 11-cis-retinol + a fatty acid | - |
? |  |
| all-trans-retinyl ester + H2O | - |
Bos taurus | 11-cis-retinol + a fatty acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61000, estimated from amino acid sequence | Homo sapiens |
| ? | x * 61000, estimated from amino acid sequence | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Rpe65 | - |
Homo sapiens |
| Rpe65 | - |
Bos taurus |
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Release 2023.1 (January 2023)
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